Review



pe conjugated anti cd86  (Elabscience Biotechnology)


Bioz Verified Symbol Elabscience Biotechnology is a verified supplier
Bioz Manufacturer Symbol Elabscience Biotechnology manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95

    Structured Review

    Elabscience Biotechnology pe conjugated anti cd86
    Pe Conjugated Anti Cd86, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 110 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pe+anti+mouse+cd86+antibody/PE+Anti-Mouse+CD86+Antibody/pmc13014926-101-18-20
    Average 95 stars, based on 110 article reviews
    pe conjugated anti cd86 - by Bioz Stars, 2026-10
    95/100 stars

    Images

    Related Articles

    other:

    Article Title: Inhibition of Breast Cancer Bone Metastasis by LRP5-Overexpressing Osteocytes via the LIMA1/MYO5B Signaling Axis
    Article Snippet: Cells were stained with Annexin V-FITC Apoptosis Detection Kit (Solarbio, CA1020, Beijing, China), then analyzed by flow cytometry.

    Article Title: Selenium-albumin corona rinse ameliorates diabetic periodontitis by inhibiting inflammation, anti-bacterial and improving osteogenesis via activating TrxR1/ROS/β-catenin anti-oxidation cascade.
    Article Snippet: Diabetes mellitus (DM) aggravates periodontitis due to disordered glucose metabolism, leading to accelerated bone destruction and increasing dental plaque reproduction to induce local inflammation.. The hyperglycemic state of DM leads to reactive oxygen species (ROS) overproduction that inhibits osteogenic differentiation in periodontal.. These make a great challenge for the treatment of diabetic periodontitis.

    Article Title: Inhibition of Breast Cancer Bone Metastasis by LRP5-Overexpressing Osteocytes via the LIMA1/MYO5B Signaling Axis.
    Article Snippet: Secreted LIMA1 levels in the supernatant were measured using a commercial ELISA kit (MyBiosource, MBS289188, San Diego, CA, USA).

    Staining:

    Article Title: A mitochondria-targeted nanomedicine for myocardial ischemia/reperfusion injury with synergistic antioxidant and anti-inflammatory properties
    Article Snippet: .. PE Anti-Mouse CD86 Antibody (Elabscience) or APC Anti-Mouse CD206/MMR Antibody (Elabscience) was used for staining, and the cells were detected by flow cytometry. .. C57BL/6 C mice (6–8 weeks, male) were obtained from Charles River Laboratories (Beijing, China) and were bred in a specific pathogen-free (SPF) environment.

    Article Title: A mitochondria-targeted nanomedicine for myocardial ischemia/reperfusion injury with synergistic antioxidant and anti-inflammatory properties.
    Article Snippet: .. PE Anti-Mouse CD86 Antibody (Elabscience) or APC Anti-Mouse CD206/ MMR Antibody (Elabscience) was used for staining, and the cells were detected by flow cytometry. .. Myocardial infarction injury model in mouse C57BL/6 C mice (6–8 weeks, male) were obtained from Charles River Laboratories (Beijing, China) and were bred in a specific pathogen-free (SPF) environment.

    Article Title: Huangtu Decoction regulates macrophage M1/M2 polarization to alleviate DSS- and rhubarb-induced Deficiency-Cold Pattern in Ulcerative Colitis by inhibiting the HIF-1α signaling pathway.
    Article Snippet: Ethnopharmacological relevance: Huangtu Decoction (HTD), a classic formula in TCM, was first documented in the Synopsis of the Golden Chamber and later included in numerous TCM texts.. This formula is commonly used to treat various gastrointestinal disorders, including diarrhea, bloody stools, irritable bowel syndrome, and ulcerative

    Flow Cytometry:

    Article Title: A mitochondria-targeted nanomedicine for myocardial ischemia/reperfusion injury with synergistic antioxidant and anti-inflammatory properties
    Article Snippet: .. PE Anti-Mouse CD86 Antibody (Elabscience) or APC Anti-Mouse CD206/MMR Antibody (Elabscience) was used for staining, and the cells were detected by flow cytometry. .. C57BL/6 C mice (6–8 weeks, male) were obtained from Charles River Laboratories (Beijing, China) and were bred in a specific pathogen-free (SPF) environment.

    Article Title: A mitochondria-targeted nanomedicine for myocardial ischemia/reperfusion injury with synergistic antioxidant and anti-inflammatory properties.
    Article Snippet: .. PE Anti-Mouse CD86 Antibody (Elabscience) or APC Anti-Mouse CD206/ MMR Antibody (Elabscience) was used for staining, and the cells were detected by flow cytometry. .. Myocardial infarction injury model in mouse C57BL/6 C mice (6–8 weeks, male) were obtained from Charles River Laboratories (Beijing, China) and were bred in a specific pathogen-free (SPF) environment.

    Article Title: Huangtu Decoction regulates macrophage M1/M2 polarization to alleviate DSS- and rhubarb-induced Deficiency-Cold Pattern in Ulcerative Colitis by inhibiting the HIF-1α signaling pathway.
    Article Snippet: Ethnopharmacological relevance: Huangtu Decoction (HTD), a classic formula in TCM, was first documented in the Synopsis of the Golden Chamber and later included in numerous TCM texts.. This formula is commonly used to treat various gastrointestinal disorders, including diarrhea, bloody stools, irritable bowel syndrome, and ulcerative

    Purification:

    Article Title: Arsenic trioxide-based nanoparticles for enhanced chemotherapy by activating pyroptosis
    Article Snippet: Mouse tumor necrosis factor α (TNF- α ) ELISA kit, mouse interleukin 6 (IL-6) ELISA kit, mouse interleukin 1 β (IL-1 β ) ELISA kit instruction and mouse high mobility group protein B1(HMGB1) were from MEIMIAN (Wuhan, China). .. APC Anti-Mouse CD11c Antibody, PE Anti-Mouse CD86 Antibody, FITC Anti-Mouse CD3 Antibody, APC Anti-Mouse CD4 Antibody, PE Anti-Mouse CD8a Antibody and Purified Anti-Mouse CD16/32 Antibody were from Elabscience (Wuhan, China). .. Hepa1-6 cells were obtained from Meisen CTCC (Jinhua, China).

    Article Title: Huangtu Decoction regulates macrophage M1/M2 polarization to alleviate DSS- and rhubarb-induced Deficiency-Cold Pattern in Ulcerative Colitis by inhibiting the HIF-1α signaling pathway.
    Article Snippet: Ethnopharmacological relevance: Huangtu Decoction (HTD), a classic formula in TCM, was first documented in the Synopsis of the Golden Chamber and later included in numerous TCM texts.. This formula is commonly used to treat various gastrointestinal disorders, including diarrhea, bloody stools, irritable bowel syndrome, and ulcerative

    Article Title: Intravesical folate-conjugated hydroxyethyl starch micelles for pH-triggered co-delivery of epirubicin and TLR7 agonist toward synergistic chemoimmunotherapy of bladder cancer
    Article Snippet: Imiquimod (IMQ) was purchased from TCI (Tokyo, Japan). .. Rabbit anti-CD86 polyclonal antibody, rabbit anti-CD206 polyclonal antibody, FITC goat anti-rabbit antibody, IgG/Alexa Fluor555 goat anti-rabbit antibody, APC anti-mouse CD206 antibody, PE anti-mouse CD86 antibody, purified anti-mouse CD16/32 antibody, intracellular Fixation/Permeabilization buffer kit, IL-6 ELISA kit and TNF-α ELISA kit were all purchased from Elabscience Biotechnology (Wuhan, China). .. IL-4 was purchased from Biyuntian Biotechnology (Shanghai, China).

    Immunofluorescence:

    Article Title: Huangtu Decoction regulates macrophage M1/M2 polarization to alleviate DSS- and rhubarb-induced Deficiency-Cold Pattern in Ulcerative Colitis by inhibiting the HIF-1α signaling pathway.
    Article Snippet: Ethnopharmacological relevance: Huangtu Decoction (HTD), a classic formula in TCM, was first documented in the Synopsis of the Golden Chamber and later included in numerous TCM texts.. This formula is commonly used to treat various gastrointestinal disorders, including diarrhea, bloody stools, irritable bowel syndrome, and ulcerative

    Western Blot:

    Article Title: Huangtu Decoction regulates macrophage M1/M2 polarization to alleviate DSS- and rhubarb-induced Deficiency-Cold Pattern in Ulcerative Colitis by inhibiting the HIF-1α signaling pathway.
    Article Snippet: Ethnopharmacological relevance: Huangtu Decoction (HTD), a classic formula in TCM, was first documented in the Synopsis of the Golden Chamber and later included in numerous TCM texts.. This formula is commonly used to treat various gastrointestinal disorders, including diarrhea, bloody stools, irritable bowel syndrome, and ulcerative

    Recombinant:

    Article Title: Huangtu Decoction regulates macrophage M1/M2 polarization to alleviate DSS- and rhubarb-induced Deficiency-Cold Pattern in Ulcerative Colitis by inhibiting the HIF-1α signaling pathway.
    Article Snippet: Ethnopharmacological relevance: Huangtu Decoction (HTD), a classic formula in TCM, was first documented in the Synopsis of the Golden Chamber and later included in numerous TCM texts.. This formula is commonly used to treat various gastrointestinal disorders, including diarrhea, bloody stools, irritable bowel syndrome, and ulcerative

    Enzyme-linked Immunosorbent Assay:

    Article Title: Intravesical folate-conjugated hydroxyethyl starch micelles for pH-triggered co-delivery of epirubicin and TLR7 agonist toward synergistic chemoimmunotherapy of bladder cancer
    Article Snippet: Imiquimod (IMQ) was purchased from TCI (Tokyo, Japan). .. Rabbit anti-CD86 polyclonal antibody, rabbit anti-CD206 polyclonal antibody, FITC goat anti-rabbit antibody, IgG/Alexa Fluor555 goat anti-rabbit antibody, APC anti-mouse CD206 antibody, PE anti-mouse CD86 antibody, purified anti-mouse CD16/32 antibody, intracellular Fixation/Permeabilization buffer kit, IL-6 ELISA kit and TNF-α ELISA kit were all purchased from Elabscience Biotechnology (Wuhan, China). .. IL-4 was purchased from Biyuntian Biotechnology (Shanghai, China).



    Similar Products

    95
    Elabscience Biotechnology pe conjugated anti cd86
    Pe Conjugated Anti Cd86, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pe+anti+mouse+cd86+antibody/PE+Anti-Mouse+CD86+Antibody/pmc13014926-101-18-20
    Average 95 stars, based on 1 article reviews
    pe conjugated anti cd86 - by Bioz Stars, 2026-10
    95/100 stars
      Buy from Supplier

    95
    Elabscience Biotechnology pe anti mouse cd86 antibody
    (A) CLSM images of RAW 264.7 cells after treatment with LPS + IFN-γ and IL-4. Cell nuclei were stained with DAPI (blue), <t>CD86</t> fluorescence displayed in green and CD206 fluorescence displayed in red. Scale bar: 10 μm; (B) Confocal imaging of CD86 and CD206 expression in M2-TAMs treated with free IMQ, FA-HES-EPI, and FA-HES-EPI/IMQ micelles. Cell nuclei were stained with DAPI (blue), CD86 fluorescence displayed in green and CD206 fluorescence displayed in red. Scale bar: 10 μm; (C) FCM analysis of expression of CD86 and CD206 after co-culturing M2-TAMs with different formulations; (D) Mean fluorescence intensity of CD86 and CD206 in M2-TAMs treated with different formulations. (n = 3), n.s.: p > 0.05, **p < 0.01and ****p < 0.0001 vs. the IL-4 group; ##p < 0.01and ####p < 0.0001 vs. the IL-4 group; (E) Expression levels of IL-6 and TNF-α in RAW264.7 cells treated with free IMQ and FA-HES-EPI/IMQ micelles. (n = 3), n.s.: p > 0.05, ***p < 0.001 and ****p < 0.0001; ####p < 0.0001; (F) Western blot analysis of TLR7/MyD88/NF-κB signaling in RAW 264.7 cells treated with free IMQ or FA-HES-EPI/IMQ micelles.
    Pe Anti Mouse Cd86 Antibody, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pe+anti+mouse+cd86+antibody/PE+Anti-Mouse+CD86+Antibody/pmc12859460-48-21-44
    Average 95 stars, based on 1 article reviews
    pe anti mouse cd86 antibody - by Bioz Stars, 2026-10
    95/100 stars
      Buy from Supplier

    95
    Elabscience Biotechnology anti mouse mhcii
    Impact of gonococcal OMVs on dendritic cell activation and T cell differentiation. Mature murine bone marrow-derived dendritic cells (BMDCs) were stimulated with OMV-PorB WT , OMV-PorB K117Q/K171Q or a Mock control and surface expression of CD86 and <t>MHCII</t> was determined by flow cytometry. Stimulated BMDCs were subsequently co-cultured with naïve murine T cells and IFN-γ, TNF-α, and IL-4 cytokine levels in the culture supernatant was determined by ELISA. (A) Representative flow cytometry repeat for detection of CD86 levels at the surface of OMV-stimulated DCs. (B) Quantification of CD86 levels at the surface of OMV-stimulated DCs. (C) Representative flow cytometry repeat for detection of MHCII levels at the surface of OMV-stimulated DCs. (D) Quantification of MHCII levels at the surface of OMV-stimulated DCs. (E) Quantification of IL-2 secretion in the culture supernatant after co-culture of T cells with OMV-stimulated DCs. (F) Quantification of IL-10 secretion in the culture supernatant after co-culture of T cells with OMV-stimulated DCs. (G) Quantification of IL-4 secretion in the culture supernatant after co-culture of T cells with OMV-stimulated DCs. (H) Quantification of IFN-γ secretion in the culture supernatant after co-culture of T cells with OMV-stimulated DCs. (I) Quantification of TNF-α secretion in the culture supernatant after co-culture of T cells with OMV-stimulated DCs. (J) Quantification of IL-17A secretion in the culture supernatant after co-culture of T cells with OMV-stimulated DCs. Graphs represent mean ± SD of three biological repeats. Significant differences were identified by one-way ANOVA (GraphPad Prism).
    Anti Mouse Mhcii, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pe+anti+mouse+cd86+antibody/PE+Anti-Mouse+CD86+Antibody/pmc13063334-51-55-57
    Average 95 stars, based on 1 article reviews
    anti mouse mhcii - by Bioz Stars, 2026-10
    95/100 stars
      Buy from Supplier

    95
    Elabscience Biotechnology anti mouse cd86
    Impact of gonococcal OMVs on dendritic cell activation and T cell differentiation. Mature murine bone marrow-derived dendritic cells (BMDCs) were stimulated with OMV-PorB WT , OMV-PorB K117Q/K171Q or a Mock control and surface expression of CD86 and <t>MHCII</t> was determined by flow cytometry. Stimulated BMDCs were subsequently co-cultured with naïve murine T cells and IFN-γ, TNF-α, and IL-4 cytokine levels in the culture supernatant was determined by ELISA. (A) Representative flow cytometry repeat for detection of CD86 levels at the surface of OMV-stimulated DCs. (B) Quantification of CD86 levels at the surface of OMV-stimulated DCs. (C) Representative flow cytometry repeat for detection of MHCII levels at the surface of OMV-stimulated DCs. (D) Quantification of MHCII levels at the surface of OMV-stimulated DCs. (E) Quantification of IL-2 secretion in the culture supernatant after co-culture of T cells with OMV-stimulated DCs. (F) Quantification of IL-10 secretion in the culture supernatant after co-culture of T cells with OMV-stimulated DCs. (G) Quantification of IL-4 secretion in the culture supernatant after co-culture of T cells with OMV-stimulated DCs. (H) Quantification of IFN-γ secretion in the culture supernatant after co-culture of T cells with OMV-stimulated DCs. (I) Quantification of TNF-α secretion in the culture supernatant after co-culture of T cells with OMV-stimulated DCs. (J) Quantification of IL-17A secretion in the culture supernatant after co-culture of T cells with OMV-stimulated DCs. Graphs represent mean ± SD of three biological repeats. Significant differences were identified by one-way ANOVA (GraphPad Prism).
    Anti Mouse Cd86, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pe+anti+mouse+cd86+antibody/PE+Anti-Mouse+CD86+Antibody/10__1080_slash_22221751__2026__2651468-63-20-22
    Average 95 stars, based on 1 article reviews
    anti mouse cd86 - by Bioz Stars, 2026-10
    95/100 stars
      Buy from Supplier

    95
    Elabscience Biotechnology apc
    Impact of gonococcal OMVs on dendritic cell activation and T cell differentiation. Mature murine bone marrow-derived dendritic cells (BMDCs) were stimulated with OMV-PorB WT , OMV-PorB K117Q/K171Q or a Mock control and surface expression of CD86 and <t>MHCII</t> was determined by flow cytometry. Stimulated BMDCs were subsequently co-cultured with naïve murine T cells and IFN-γ, TNF-α, and IL-4 cytokine levels in the culture supernatant was determined by ELISA. (A) Representative flow cytometry repeat for detection of CD86 levels at the surface of OMV-stimulated DCs. (B) Quantification of CD86 levels at the surface of OMV-stimulated DCs. (C) Representative flow cytometry repeat for detection of MHCII levels at the surface of OMV-stimulated DCs. (D) Quantification of MHCII levels at the surface of OMV-stimulated DCs. (E) Quantification of IL-2 secretion in the culture supernatant after co-culture of T cells with OMV-stimulated DCs. (F) Quantification of IL-10 secretion in the culture supernatant after co-culture of T cells with OMV-stimulated DCs. (G) Quantification of IL-4 secretion in the culture supernatant after co-culture of T cells with OMV-stimulated DCs. (H) Quantification of IFN-γ secretion in the culture supernatant after co-culture of T cells with OMV-stimulated DCs. (I) Quantification of TNF-α secretion in the culture supernatant after co-culture of T cells with OMV-stimulated DCs. (J) Quantification of IL-17A secretion in the culture supernatant after co-culture of T cells with OMV-stimulated DCs. Graphs represent mean ± SD of three biological repeats. Significant differences were identified by one-way ANOVA (GraphPad Prism).
    Apc, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pe+anti+mouse+cd86+antibody/PE+Anti-Mouse+CD86+Antibody/pmc13063334-51-54-57
    Average 95 stars, based on 1 article reviews
    apc - by Bioz Stars, 2026-10
    95/100 stars
      Buy from Supplier

    94
    Elabscience Biotechnology cyanine7 anti mouse cd86 antibody
    Impact of gonococcal OMVs on dendritic cell activation and T cell differentiation. Mature murine bone marrow-derived dendritic cells (BMDCs) were stimulated with OMV-PorB WT , OMV-PorB K117Q/K171Q or a Mock control and surface expression of CD86 and <t>MHCII</t> was determined by flow cytometry. Stimulated BMDCs were subsequently co-cultured with naïve murine T cells and IFN-γ, TNF-α, and IL-4 cytokine levels in the culture supernatant was determined by ELISA. (A) Representative flow cytometry repeat for detection of CD86 levels at the surface of OMV-stimulated DCs. (B) Quantification of CD86 levels at the surface of OMV-stimulated DCs. (C) Representative flow cytometry repeat for detection of MHCII levels at the surface of OMV-stimulated DCs. (D) Quantification of MHCII levels at the surface of OMV-stimulated DCs. (E) Quantification of IL-2 secretion in the culture supernatant after co-culture of T cells with OMV-stimulated DCs. (F) Quantification of IL-10 secretion in the culture supernatant after co-culture of T cells with OMV-stimulated DCs. (G) Quantification of IL-4 secretion in the culture supernatant after co-culture of T cells with OMV-stimulated DCs. (H) Quantification of IFN-γ secretion in the culture supernatant after co-culture of T cells with OMV-stimulated DCs. (I) Quantification of TNF-α secretion in the culture supernatant after co-culture of T cells with OMV-stimulated DCs. (J) Quantification of IL-17A secretion in the culture supernatant after co-culture of T cells with OMV-stimulated DCs. Graphs represent mean ± SD of three biological repeats. Significant differences were identified by one-way ANOVA (GraphPad Prism).
    Cyanine7 Anti Mouse Cd86 Antibody, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pe+anti+mouse+cd86+antibody/PE%2FCyanine7+Anti-Mouse+CD86+Antibody/pm41839266-63-32-37
    Average 94 stars, based on 1 article reviews
    cyanine7 anti mouse cd86 antibody - by Bioz Stars, 2026-10
    94/100 stars
      Buy from Supplier

    95
    Elabscience Biotechnology cd86 pe
    Impact of gonococcal OMVs on dendritic cell activation and T cell differentiation. Mature murine bone marrow-derived dendritic cells (BMDCs) were stimulated with OMV-PorB WT , OMV-PorB K117Q/K171Q or a Mock control and surface expression of CD86 and <t>MHCII</t> was determined by flow cytometry. Stimulated BMDCs were subsequently co-cultured with naïve murine T cells and IFN-γ, TNF-α, and IL-4 cytokine levels in the culture supernatant was determined by ELISA. (A) Representative flow cytometry repeat for detection of CD86 levels at the surface of OMV-stimulated DCs. (B) Quantification of CD86 levels at the surface of OMV-stimulated DCs. (C) Representative flow cytometry repeat for detection of MHCII levels at the surface of OMV-stimulated DCs. (D) Quantification of MHCII levels at the surface of OMV-stimulated DCs. (E) Quantification of IL-2 secretion in the culture supernatant after co-culture of T cells with OMV-stimulated DCs. (F) Quantification of IL-10 secretion in the culture supernatant after co-culture of T cells with OMV-stimulated DCs. (G) Quantification of IL-4 secretion in the culture supernatant after co-culture of T cells with OMV-stimulated DCs. (H) Quantification of IFN-γ secretion in the culture supernatant after co-culture of T cells with OMV-stimulated DCs. (I) Quantification of TNF-α secretion in the culture supernatant after co-culture of T cells with OMV-stimulated DCs. (J) Quantification of IL-17A secretion in the culture supernatant after co-culture of T cells with OMV-stimulated DCs. Graphs represent mean ± SD of three biological repeats. Significant differences were identified by one-way ANOVA (GraphPad Prism).
    Cd86 Pe, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pe+anti+mouse+cd86+antibody/PE+Anti-Mouse+CD86+Antibody/pm41818620-54-6-14
    Average 95 stars, based on 1 article reviews
    cd86 pe - by Bioz Stars, 2026-10
    95/100 stars
      Buy from Supplier

    95
    Elabscience Biotechnology anti cd86 pe
    Impact of gonococcal OMVs on dendritic cell activation and T cell differentiation. Mature murine bone marrow-derived dendritic cells (BMDCs) were stimulated with OMV-PorB WT , OMV-PorB K117Q/K171Q or a Mock control and surface expression of CD86 and <t>MHCII</t> was determined by flow cytometry. Stimulated BMDCs were subsequently co-cultured with naïve murine T cells and IFN-γ, TNF-α, and IL-4 cytokine levels in the culture supernatant was determined by ELISA. (A) Representative flow cytometry repeat for detection of CD86 levels at the surface of OMV-stimulated DCs. (B) Quantification of CD86 levels at the surface of OMV-stimulated DCs. (C) Representative flow cytometry repeat for detection of MHCII levels at the surface of OMV-stimulated DCs. (D) Quantification of MHCII levels at the surface of OMV-stimulated DCs. (E) Quantification of IL-2 secretion in the culture supernatant after co-culture of T cells with OMV-stimulated DCs. (F) Quantification of IL-10 secretion in the culture supernatant after co-culture of T cells with OMV-stimulated DCs. (G) Quantification of IL-4 secretion in the culture supernatant after co-culture of T cells with OMV-stimulated DCs. (H) Quantification of IFN-γ secretion in the culture supernatant after co-culture of T cells with OMV-stimulated DCs. (I) Quantification of TNF-α secretion in the culture supernatant after co-culture of T cells with OMV-stimulated DCs. (J) Quantification of IL-17A secretion in the culture supernatant after co-culture of T cells with OMV-stimulated DCs. Graphs represent mean ± SD of three biological repeats. Significant differences were identified by one-way ANOVA (GraphPad Prism).
    Anti Cd86 Pe, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pe+anti+mouse+cd86+antibody/PE+Anti-Mouse+CD86+Antibody/pm41812835-84-4-25
    Average 95 stars, based on 1 article reviews
    anti cd86 pe - by Bioz Stars, 2026-10
    95/100 stars
      Buy from Supplier

    Image Search Results


    (A) CLSM images of RAW 264.7 cells after treatment with LPS + IFN-γ and IL-4. Cell nuclei were stained with DAPI (blue), CD86 fluorescence displayed in green and CD206 fluorescence displayed in red. Scale bar: 10 μm; (B) Confocal imaging of CD86 and CD206 expression in M2-TAMs treated with free IMQ, FA-HES-EPI, and FA-HES-EPI/IMQ micelles. Cell nuclei were stained with DAPI (blue), CD86 fluorescence displayed in green and CD206 fluorescence displayed in red. Scale bar: 10 μm; (C) FCM analysis of expression of CD86 and CD206 after co-culturing M2-TAMs with different formulations; (D) Mean fluorescence intensity of CD86 and CD206 in M2-TAMs treated with different formulations. (n = 3), n.s.: p > 0.05, **p < 0.01and ****p < 0.0001 vs. the IL-4 group; ##p < 0.01and ####p < 0.0001 vs. the IL-4 group; (E) Expression levels of IL-6 and TNF-α in RAW264.7 cells treated with free IMQ and FA-HES-EPI/IMQ micelles. (n = 3), n.s.: p > 0.05, ***p < 0.001 and ****p < 0.0001; ####p < 0.0001; (F) Western blot analysis of TLR7/MyD88/NF-κB signaling in RAW 264.7 cells treated with free IMQ or FA-HES-EPI/IMQ micelles.

    Journal: Materials Today Bio

    Article Title: Intravesical folate-conjugated hydroxyethyl starch micelles for pH-triggered co-delivery of epirubicin and TLR7 agonist toward synergistic chemoimmunotherapy of bladder cancer

    doi: 10.1016/j.mtbio.2026.102835

    Figure Lengend Snippet: (A) CLSM images of RAW 264.7 cells after treatment with LPS + IFN-γ and IL-4. Cell nuclei were stained with DAPI (blue), CD86 fluorescence displayed in green and CD206 fluorescence displayed in red. Scale bar: 10 μm; (B) Confocal imaging of CD86 and CD206 expression in M2-TAMs treated with free IMQ, FA-HES-EPI, and FA-HES-EPI/IMQ micelles. Cell nuclei were stained with DAPI (blue), CD86 fluorescence displayed in green and CD206 fluorescence displayed in red. Scale bar: 10 μm; (C) FCM analysis of expression of CD86 and CD206 after co-culturing M2-TAMs with different formulations; (D) Mean fluorescence intensity of CD86 and CD206 in M2-TAMs treated with different formulations. (n = 3), n.s.: p > 0.05, **p < 0.01and ****p < 0.0001 vs. the IL-4 group; ##p < 0.01and ####p < 0.0001 vs. the IL-4 group; (E) Expression levels of IL-6 and TNF-α in RAW264.7 cells treated with free IMQ and FA-HES-EPI/IMQ micelles. (n = 3), n.s.: p > 0.05, ***p < 0.001 and ****p < 0.0001; ####p < 0.0001; (F) Western blot analysis of TLR7/MyD88/NF-κB signaling in RAW 264.7 cells treated with free IMQ or FA-HES-EPI/IMQ micelles.

    Article Snippet: Rabbit anti-CD86 polyclonal antibody, rabbit anti-CD206 polyclonal antibody, FITC goat anti-rabbit antibody, IgG/Alexa Fluor555 goat anti-rabbit antibody, APC anti-mouse CD206 antibody, PE anti-mouse CD86 antibody, purified anti-mouse CD16/32 antibody, intracellular Fixation/Permeabilization buffer kit, IL-6 ELISA kit and TNF-α ELISA kit were all purchased from Elabscience Biotechnology (Wuhan, China).

    Techniques: Staining, Fluorescence, Imaging, Expressing, Western Blot

    Impact of gonococcal OMVs on dendritic cell activation and T cell differentiation. Mature murine bone marrow-derived dendritic cells (BMDCs) were stimulated with OMV-PorB WT , OMV-PorB K117Q/K171Q or a Mock control and surface expression of CD86 and MHCII was determined by flow cytometry. Stimulated BMDCs were subsequently co-cultured with naïve murine T cells and IFN-γ, TNF-α, and IL-4 cytokine levels in the culture supernatant was determined by ELISA. (A) Representative flow cytometry repeat for detection of CD86 levels at the surface of OMV-stimulated DCs. (B) Quantification of CD86 levels at the surface of OMV-stimulated DCs. (C) Representative flow cytometry repeat for detection of MHCII levels at the surface of OMV-stimulated DCs. (D) Quantification of MHCII levels at the surface of OMV-stimulated DCs. (E) Quantification of IL-2 secretion in the culture supernatant after co-culture of T cells with OMV-stimulated DCs. (F) Quantification of IL-10 secretion in the culture supernatant after co-culture of T cells with OMV-stimulated DCs. (G) Quantification of IL-4 secretion in the culture supernatant after co-culture of T cells with OMV-stimulated DCs. (H) Quantification of IFN-γ secretion in the culture supernatant after co-culture of T cells with OMV-stimulated DCs. (I) Quantification of TNF-α secretion in the culture supernatant after co-culture of T cells with OMV-stimulated DCs. (J) Quantification of IL-17A secretion in the culture supernatant after co-culture of T cells with OMV-stimulated DCs. Graphs represent mean ± SD of three biological repeats. Significant differences were identified by one-way ANOVA (GraphPad Prism).

    Journal: Emerging Microbes & Infections

    Article Title: Abolishing PorB-induced mitophagy enhances gonococcal outer membrane vesicle vaccine efficacy

    doi: 10.1080/22221751.2026.2651468

    Figure Lengend Snippet: Impact of gonococcal OMVs on dendritic cell activation and T cell differentiation. Mature murine bone marrow-derived dendritic cells (BMDCs) were stimulated with OMV-PorB WT , OMV-PorB K117Q/K171Q or a Mock control and surface expression of CD86 and MHCII was determined by flow cytometry. Stimulated BMDCs were subsequently co-cultured with naïve murine T cells and IFN-γ, TNF-α, and IL-4 cytokine levels in the culture supernatant was determined by ELISA. (A) Representative flow cytometry repeat for detection of CD86 levels at the surface of OMV-stimulated DCs. (B) Quantification of CD86 levels at the surface of OMV-stimulated DCs. (C) Representative flow cytometry repeat for detection of MHCII levels at the surface of OMV-stimulated DCs. (D) Quantification of MHCII levels at the surface of OMV-stimulated DCs. (E) Quantification of IL-2 secretion in the culture supernatant after co-culture of T cells with OMV-stimulated DCs. (F) Quantification of IL-10 secretion in the culture supernatant after co-culture of T cells with OMV-stimulated DCs. (G) Quantification of IL-4 secretion in the culture supernatant after co-culture of T cells with OMV-stimulated DCs. (H) Quantification of IFN-γ secretion in the culture supernatant after co-culture of T cells with OMV-stimulated DCs. (I) Quantification of TNF-α secretion in the culture supernatant after co-culture of T cells with OMV-stimulated DCs. (J) Quantification of IL-17A secretion in the culture supernatant after co-culture of T cells with OMV-stimulated DCs. Graphs represent mean ± SD of three biological repeats. Significant differences were identified by one-way ANOVA (GraphPad Prism).

    Article Snippet: Mature DCs (1 × 106 cells/well) were stimulated for 20 h with 20 μg of OMV-PorB WT or OMV-PorB K117Q/K171Q or with a PBS-only Mock control. for 20 h. Expression of CD86 and MHCII was analyzed by flow cytometry on CD11c-positive cells (FITC-labeled anti-mouse CD11c; Elabscience, #E-AB-F0991C) using PE-labeled anti-mouse CD86 (Elabscience, #E-AB-F0994D) and APC-labeled anti-mouse MHCII (Elabscience, #E-AB-F0990E).

    Techniques: Activation Assay, Cell Differentiation, Derivative Assay, Control, Expressing, Flow Cytometry, Cell Culture, Enzyme-linked Immunosorbent Assay, Co-Culture Assay